Control:Article Title: Machine Learning-Assisted Rapid Optical Imaging for Label-Free CAR T-Cell Detection in Whole Blood
Article Snippet: .. As depicted in Figure S1, the sample delivery and detection system consists of four parts: (1) Flow control—Syringe pump (Chemyx Fusion 100, Chemyx Inc., Stafford, TX, USA, Catalog #: 07100) provides constant flow rate (10 μL/min); 6-port injection valve (VICI Valco Instruments Co. Inc., Houston, TX, USA, #08N-0232H) for switching between washing buffer and the fluorescent protein; 20 mL Sterile syringe for loading washing buffer; and 1 mL sterile syringe for loading cells samples (VWR # 53548-008 and 53548-001, VWR International, Radnor, PA, USA). (2) Sensor chip—Homemade microfluidic chips with a straight channel and a CD19-functionalized surface for CAR T-cell capture and imaging. (3) Image acquisition—An inverted microscope (Olympus IX81, Olympus Corporation of the Americas, Center Valley, PA, USA, 40×/0.75 NA objective) with a CMOS camera for recording brightfield and fluorescence image sequences. (4) Data processing—Digital cellular crowding was reduced through a custom Python 3.9 script; CAR T-cells were identified and quantified with ilastik, a machine learning-based image processing toolkit. ..
Injection:Article Title: Machine Learning-Assisted Rapid Optical Imaging for Label-Free CAR T-Cell Detection in Whole Blood
Article Snippet: .. As depicted in Figure S1, the sample delivery and detection system consists of four parts: (1) Flow control—Syringe pump (Chemyx Fusion 100, Chemyx Inc., Stafford, TX, USA, Catalog #: 07100) provides constant flow rate (10 μL/min); 6-port injection valve (VICI Valco Instruments Co. Inc., Houston, TX, USA, #08N-0232H) for switching between washing buffer and the fluorescent protein; 20 mL Sterile syringe for loading washing buffer; and 1 mL sterile syringe for loading cells samples (VWR # 53548-008 and 53548-001, VWR International, Radnor, PA, USA). (2) Sensor chip—Homemade microfluidic chips with a straight channel and a CD19-functionalized surface for CAR T-cell capture and imaging. (3) Image acquisition—An inverted microscope (Olympus IX81, Olympus Corporation of the Americas, Center Valley, PA, USA, 40×/0.75 NA objective) with a CMOS camera for recording brightfield and fluorescence image sequences. (4) Data processing—Digital cellular crowding was reduced through a custom Python 3.9 script; CAR T-cells were identified and quantified with ilastik, a machine learning-based image processing toolkit. ..
Sterility:Article Title: Machine Learning-Assisted Rapid Optical Imaging for Label-Free CAR T-Cell Detection in Whole Blood
Article Snippet: .. As depicted in Figure S1, the sample delivery and detection system consists of four parts: (1) Flow control—Syringe pump (Chemyx Fusion 100, Chemyx Inc., Stafford, TX, USA, Catalog #: 07100) provides constant flow rate (10 μL/min); 6-port injection valve (VICI Valco Instruments Co. Inc., Houston, TX, USA, #08N-0232H) for switching between washing buffer and the fluorescent protein; 20 mL Sterile syringe for loading washing buffer; and 1 mL sterile syringe for loading cells samples (VWR # 53548-008 and 53548-001, VWR International, Radnor, PA, USA). (2) Sensor chip—Homemade microfluidic chips with a straight channel and a CD19-functionalized surface for CAR T-cell capture and imaging. (3) Image acquisition—An inverted microscope (Olympus IX81, Olympus Corporation of the Americas, Center Valley, PA, USA, 40×/0.75 NA objective) with a CMOS camera for recording brightfield and fluorescence image sequences. (4) Data processing—Digital cellular crowding was reduced through a custom Python 3.9 script; CAR T-cells were identified and quantified with ilastik, a machine learning-based image processing toolkit. ..
Imaging:Article Title: Machine Learning-Assisted Rapid Optical Imaging for Label-Free CAR T-Cell Detection in Whole Blood
Article Snippet: .. As depicted in Figure S1, the sample delivery and detection system consists of four parts: (1) Flow control—Syringe pump (Chemyx Fusion 100, Chemyx Inc., Stafford, TX, USA, Catalog #: 07100) provides constant flow rate (10 μL/min); 6-port injection valve (VICI Valco Instruments Co. Inc., Houston, TX, USA, #08N-0232H) for switching between washing buffer and the fluorescent protein; 20 mL Sterile syringe for loading washing buffer; and 1 mL sterile syringe for loading cells samples (VWR # 53548-008 and 53548-001, VWR International, Radnor, PA, USA). (2) Sensor chip—Homemade microfluidic chips with a straight channel and a CD19-functionalized surface for CAR T-cell capture and imaging. (3) Image acquisition—An inverted microscope (Olympus IX81, Olympus Corporation of the Americas, Center Valley, PA, USA, 40×/0.75 NA objective) with a CMOS camera for recording brightfield and fluorescence image sequences. (4) Data processing—Digital cellular crowding was reduced through a custom Python 3.9 script; CAR T-cells were identified and quantified with ilastik, a machine learning-based image processing toolkit. ..
Inverted Microscopy:Article Title: Machine Learning-Assisted Rapid Optical Imaging for Label-Free CAR T-Cell Detection in Whole Blood
Article Snippet: .. As depicted in Figure S1, the sample delivery and detection system consists of four parts: (1) Flow control—Syringe pump (Chemyx Fusion 100, Chemyx Inc., Stafford, TX, USA, Catalog #: 07100) provides constant flow rate (10 μL/min); 6-port injection valve (VICI Valco Instruments Co. Inc., Houston, TX, USA, #08N-0232H) for switching between washing buffer and the fluorescent protein; 20 mL Sterile syringe for loading washing buffer; and 1 mL sterile syringe for loading cells samples (VWR # 53548-008 and 53548-001, VWR International, Radnor, PA, USA). (2) Sensor chip—Homemade microfluidic chips with a straight channel and a CD19-functionalized surface for CAR T-cell capture and imaging. (3) Image acquisition—An inverted microscope (Olympus IX81, Olympus Corporation of the Americas, Center Valley, PA, USA, 40×/0.75 NA objective) with a CMOS camera for recording brightfield and fluorescence image sequences. (4) Data processing—Digital cellular crowding was reduced through a custom Python 3.9 script; CAR T-cells were identified and quantified with ilastik, a machine learning-based image processing toolkit. ..
Fluorescence:Article Title: Machine Learning-Assisted Rapid Optical Imaging for Label-Free CAR T-Cell Detection in Whole Blood
Article Snippet: .. As depicted in Figure S1, the sample delivery and detection system consists of four parts: (1) Flow control—Syringe pump (Chemyx Fusion 100, Chemyx Inc., Stafford, TX, USA, Catalog #: 07100) provides constant flow rate (10 μL/min); 6-port injection valve (VICI Valco Instruments Co. Inc., Houston, TX, USA, #08N-0232H) for switching between washing buffer and the fluorescent protein; 20 mL Sterile syringe for loading washing buffer; and 1 mL sterile syringe for loading cells samples (VWR # 53548-008 and 53548-001, VWR International, Radnor, PA, USA). (2) Sensor chip—Homemade microfluidic chips with a straight channel and a CD19-functionalized surface for CAR T-cell capture and imaging. (3) Image acquisition—An inverted microscope (Olympus IX81, Olympus Corporation of the Americas, Center Valley, PA, USA, 40×/0.75 NA objective) with a CMOS camera for recording brightfield and fluorescence image sequences. (4) Data processing—Digital cellular crowding was reduced through a custom Python 3.9 script; CAR T-cells were identified and quantified with ilastik, a machine learning-based image processing toolkit. ..
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